Pp65 antigenemia for cytomegalovirus infection diagnosis in AIDS patients
نویسندگان
چکیده
منابع مشابه
PP65 antigenemia in the diagnosis of cytomegalovirus infection in AIDS patients
Cytomegalovirus causes significant morbidity and mortality in AIDS patients and those having undergone bone marrow or another transplant. PP65 antigenemia is based on detecting viral antigen in peripheral blood leukocytes through immunochemistry and by monitoring the infection in immunocompromised individuals. The present study aimed to set up this diagnostic technique in AIDS patients with act...
متن کاملEvaluation of an abbreviated protocol for cytomegalovirus pp65 antigenemia testing.
The cytomegalovirus antigenemia assay remains a useful tool for monitoring reactivation among transplant recipients. In this study, we compared protocols using direct lysis of small-volume, whole blood (WB) samples vs peripheral blood leukocyte (PBL)-enriched fractions. Of 363 evaluable samples, 51 (14.0%) were positive by one or both methods. Sensitivity, specificity, and negative and positive...
متن کامل2-Hour cytomegalovirus pp65 antigenemia assay for rapid quantitation of cytomegalovirus in blood samples.
Of 109 blood samples tested for cytomegalovirus (CMV) antigenemia, 18 (16.5%) were positive. CMV Brite detected 13 and CMV Brite Turbo detected 16 of the 18 positives. There was no significant difference in the number of positive cells detected per sample. The seven discrepant samples contained a median of only one positive cell.
متن کاملcomparison of qualitative pcr and pp65 antigenemia for the diagnosis of cmv infection in hematopoietic stem cell transplanted patients
objectives: human cytomegalovirus (cmv) is a major life-threatening pathogen for hematopoietic stem cell transplant recipients. specific tests are used for the diagnosis and monitoring of cmv infection in transplant patients. this study evaluates the performance of pp65 antigenemia and qualitative pcr assays for monitoring cmv in such patients. methods: we analyzed 179 clinical samples from 41...
متن کاملEvaluation of a leukocyte stabilization reagent for use in the cytomegalovirus pp65 antigenemia assay.
New erythrocyte lysis and leukocyte stabilization reagents (Streck Laboratories, Inc.) were tested in the cytomegalovirus pp65 antigenemia assay, to determine if whole-blood processing time could be delayed to 24 h postdraw. The combination of these reagents gave results comparable to those for patient samples processed immediately after blood draw.
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ژورنال
عنوان ژورنال: Journal of Venomous Animals and Toxins including Tropical Diseases
سال: 2005
ISSN: 1678-9199
DOI: 10.1590/s1678-91992005000400022